Polyskope One Pathogen Detection Method

PolySkope One

PolySkope One is the first ever AOAC-approved, USDA-listed, true multiplex method for the simultaneous detection of any or all common/routine foodborne pathogens including: E. coli O157:H7, Shiga Toxin E. coli, Salmonella spp., and Listeria monocytogenes (Listeria spp. target set also available).

PolySkope is designed to simplify and reduce the cost of pathogen testing using our revolutionary novel enrichment medium (PMEM) combined with tried-and-true qPCR chemistry, a proven commodity in food safety & clinical testing. PolySkope One is a flexible and modular multiplex; routine pathogens are detected individually or simultaneously (user selectable) with one enrichment and protocol.

All other food pathogen test kits require unique and separate enrichment media, different incubation temperatures, and unique plastics & reagents for each pathogen.

The PolySkope One multiplex results in a multi-fold simplification of workflow complexity and logistics, along with a reduction of of: lab footprint, labor, training, regulatory burden, error rate, material cost of testing, plastics & disposable waste, energy consumption, and more.

In addition to the savings inherent in workflow design, the direct material costs are also extremely competitive, even compared directly to any competing single-pathogen assay kits.

PolySkope’s single protocol and shared enrichment medium also reduce the environmental impact of pathogen testing by eliminating two-thirds of plastic disposables required to test, and by reducing the energy consumed by multiple incubators at different temperatures. High-throughput labs adopting PolySkope kits can expect up to 0.74 metric ton reductions in plastic waste and 0.61 metric ton reductions in CO2 generated annually, per facility (100 samples daily).

Included in each PolySkope One Kit

100 tests each, E. coli O157:H7, Shiga Toxin E. coli, Salmonella spp., and Listeria monocytogenes* (96-well format)
PCR Amplification Mix (Master Mix)
100 tests Lysis Buffer
Positive and Negative PCR Controls
Sold Separately
- 96 well lysis block & cover
- PCR plate & plate cover
- PMEM Enrichment Media

*Listeria spp. target set also available, please specify

Low Cost, High Throughput

E. coli O157:H7, Shiga Toxin E. coli (STEC), Salmonella spp., Listeria monocytogenes (Listeria spp. target set also available)

PCR Kit

$347

PolySkope One

Multiplex q-PCR kit & plastics

List Price: $1000 / 96 sample kit

(288 tests)

Enrichment

$129

PolySkope Multiplex Enrichment Medium

(PMEM)

List Price: $425 / 2.5 Kg

(330 tests)

Total list price per test: $4.25

Advantages

High-throughput
Low cost
Reduced error (fewer steps per test)
Easy protocol
Designed with multichannel pipettors & automated liquid handling in mind
Reduced enrichment media purchased, shipped, stored, and used
Reduced qPCR kits shipped, stored and used
Reduced incubators and incubator space required
Reduced labor, prep time and run time
Reduced wet lab, equipment and storage footprint
Reduced training, protocols, and regulatory burden
Reduced environmental impact: less water, plastic, energy consumption
Easy to use, robust, & cloud capable platform with remote run control & analysis (ABI QuantStudio5 only)
Validated on a variety of instruments (ABI QuantStudio5, ABI 7500 Fast, Agilent AriaMX)

Reduce

Reduce

Enrichment media
Reagent kits
Labor for media prep, inventory, sample prep & PCR analysis
Incubator, storage & lab footprint
Equipment needed (e.g. pipettors, instruments, incubators)
Plastic disposables required (pipette tips, enrichment bags, PCR plates, etc.)
Food & water wasted
Energy consumption for storage, incubation, and analysis

Preferred Platform: ABI QuantStudio 5

PolySkope’s preferred platform is Thermo Fisher’s Applied QuantStudio™ 5 . This robust, cloud capable, and high-throughput 96-well instrument is optimized for multiplex performance and reliability, a necessity in food safety applications.

The QuantStudio™ 5 platform brings additional efficiencies for customers with multiple satellite laboratories, chief among them remote run and data analysis on instruments from any PC, tablet or smart phone. Centralized real-time monitoring and data analysis further simplifies workflow, improves efficiency, and reduces the need to maintain redundant specialized analytical personnel at every facility.

Instrumentation is provided to PolySkope customers, free of charge, with minimum orders. Maintenance and service are provided directly by the manufacturer.

PolySkope One is also available on the Agilent AriaMX and ABI 7500 Fast instruments, please specify a platform when ordering.

Quant Studio 5 brochure: Download

Validations AOAC PTM Approved

PolySkope received it’s AOAC PTM certification (pdf) in April 2018.

Currently Approved Matrices, as of April 2018

Ready-to-eat (RTE) turkey (25 g)

Baby spinach (25g)

raw ground beef (25g)

Stainless steel (4”x4” sponge)

The PolySkope One method allows the simultaneous detection of E.coli O157 STEC, Salmonella spp. and Listeria monocytogenes in food products previously enriched by culture in Polyskope Multiplex Enrichment Media (PMEM). It includes the following 4 main steps: 1) sample enrichment; 2) DNA extraction; 3) PCR and 4) data analysis and interpretation. We have tested Polyskope 1.0 in several matrices such as spices, fresh cheese, dairy, ready-to-eat deli meat, smoked salmon and peanut butter to demonstrate the wide range of efficacy of our assay.

We have also conducted a number of validation studies designed to compare the efficacy of Polyskope 1.0 to the standard reference detection methods found in the United States

PolySkope is happy to add matrices, sample sizes, and targets to its AOAC Certificate for our customers via AOAC extension applications. Internal validations have already been performed on a large variety of food matrices. PolySkope welcomes validations in challenging matrices.

Internal validations already performed