R & D

The Polyskope 1.0 pathogen detection assay utilizes the rapid, sensitive, and highly specific Real Time PCR detection technology. This kit is intended to detect the “Big Three” foodborne pathogens responsible for the majority of foodborne illness and outbreaks: Shiga Toxin Escherichia coli (STEC), Salmonella spp. and Listeria monocytogenes. Our technology was designed from the ground up to be utilized in real world ISO 17025 food laboratories and in a wide array of food matrices, while maintaining a high level of specificity in order to reduce the number of false positives. Due to our novel enrichment and lysis systems, the Polyskope 1.0 kit allows the simultaneous detection of any, or all three, of the major pathogens in a single overnight enrichment, lysis, and PCR reaction. This directly results in a 3-fold reduction in labor, media/reagent costs, regulatory burden, sample prep, media prep, laboratory space required, disposables used, and equipment required while still delivering results in 24 hours.

Our founders and staff are scientists from a variety of backgrounds, with proven track records in food safety, academic research, clinical research, and molecular diagnostics. Every member of our research staff has patents in the field; clinical molecular tests they developed are being sold worldwide.

The Polyskope 1.0 kit was designed from the beginning as a modular, multiplex RT-PCR reaction to ensure robustness and reliability. Our kit utilizes a set of highly optimized multiplex primers and probes which incorporate Taqman chemistry to ensure reliability and eliminate cross-reactions (false positives) in a range of applications. Our multiplex reaction provides an internal inhibition control which is amplified in parallel to the target DNA to guarantee a reliable result.


Informed by our test-bed ISO 17025 food safety facility in Oklahoma City, Polyskope 1.0 was developed with both end-user requirements and preferences in mind. Polyskope 1.0 is designed to be a flexible and powerful pathogen detection system. Up to 94 reactions with five pathogenic targets each can be run on the Quantstudio 5 96-well instrument. Additionally, the kit is modular; the end user can choose at the instrument analysis stage to detect a single target or all targets should the need arise. Polyskope 1.0 does not have any slow, bottleneck steps (e.g. centrifugation) allowing this assay to be used in any size laboratory. Furthermore, it has been designed from the start to be used in conjunction with multichannel micro-pipettors and automation systems to guarantee the highest-throughput and minimal technician labor.


The ABI QuantStudio platform (by ThermoFisher) serves as the Polyskope 1.0 preferred platform. This new instrument is optimized performance and reliability with multiplex reactions and offers 96 and 384 well format (offered by PolySkope in Q4 2017). The QuantStudio platform following benefits: Low Cost, reliability, Cloud and local data storage, remote analysis and run from any tablet, computer, or phone. edge PCR software and analyses to ensure the most accurate and complete result possible.

 MAIN PHONE

(405) 633-0540

 MAIN EMAIL

info@polyskopelabs.com

 MAIN EMAIL

info@polyskopelabs.com