Polyskope 1.0 Pathogen Detection Method

1 Kit. 1 Media. 1 Protocol.

Detect Shiga Toxin E coli (STEC), Salmonella spp.
and Listeria monocytogenes with a single kit & enrichment media.

The Polyskope 1.0 pathogen detection method utilizes the rapid, sensitive, and highly specific Real Time PCR detection technology. This kit is intended to detect the “Big Three” foodborne pathogens responsible for the majority of foodborne illness and outbreaks: Shiga Toxin Escherichia coli (STEC), Salmonella spp. and Listeria monocytogenes. Our technology was designed from the ground up to be utilized in real world ISO 17025 food laboratories and in a wide array of food matrices, while maintaining a high level of specificity in order to reduce the number of false positives. Due to our novel enrichment and lysis systems, the Polyskope 1.0 kit allows the simultaneous detection of any, or all three, of the major pathogens in a single overnight enrichment, lysis, and PCR reaction. This directly results in a 3-fold reduction in labor, media/reagent costs, regulatory burden, sample prep, media prep, laboratory space required, disposables used, and equipment required while still delivering results in 24 hours.


  • Lysis Buffer for DNA extraction from sample
  • PCR Amplification Mix (Master Mix)
  • Fluorescent probes specific to each virulence factor target (E. coli STEC, Salmonella spp., & Listeria mono.
  • Positive and Negative PCR Controls
  • Lysis plate and pierceable plate cover (96-Well format)
  • PCR plate & optical grade plate cover (96 Well format)

Some of the advantages of PolySkope 1.0 are:

  • 3 fold reduction in enrichment media and incubator space required (single enrichment)
  • 3 fold reduction in labor
  • 3 fold reduction in prep time
  • 3 fold reduction in assay run time
  • 3 fold reduction in equipment required (pipettors, RT-PCR instruments, etc)
  • 3 fold reduction in disposables required (pipette tips, enrichment bags, PCR plates, etc)
  • 3 fold reduction in RT-PCR reagents
  • Rapid (24 hours to results)
  • High-Throughput (96-well format)
  • Easy to use and open platform

The ABI QuantStudio platofrm (by ThermoFisher) serves as the Polyskope 1.0 dedicated platform. This new instrument is optimized performance and reliability with multiplex reactions and offers 96 and 384 well format (offered by PolySkope in Q4 2017). The QuantStudio platform following benefits: Low Cost, reliability, Cloud and local data storage, remote analysis and run from any tablet, computer, or phone. edge PCR software and analyses to ensure the most accurate and complete result possible.

AOAC PTM PENDING – expected Q3 2017

The PolySkope 1.0 method allows the simultaneous detection of E.coli O157 STEC, Salmonella spp. and Listeria monocytogenes in food products previously enriched by culture in Polyskope Multiplex Enrichment Media (PMEM). It includes the following 4 main steps: 1) sample enrichment; 2) DNA extraction; 3) PCR and 4) data analysis and interpretation. We have tested Polyskope 1.0 in several matrices such as spices, fresh cheese, dairy, ready-to-eat deli meat, smoked salmon and peanut butter to demonstrate the wide range of efficacy of our assay.

We have also conducted a number of validation studies designed to compare the efficacy of Polyskope 1.0 to the standard reference detection methods found in the United States Food and Drug Administration Bacteriological Analytical Manual (BAM), and the United States Department of Agriculture (USDA) Microbiological Laboratory Guidebook (MLG). Validation studies are available for viewing in the website documents section

The Polyskope 1.0 pathogen detection assay utilizes the rapid, sensitive, and highly specific Real Time PCR detection technology. This kit is intended to detect the “Big Three” foodborne pathogens responsible for the majority of foodborne illness and outbreaks: Shiga Toxin Escherichia coli (STEC), Salmonella spp. and Listeria monocytogenes. Our technology was designed from the ground up to be utilized in real world ISO 17025 food laboratories and in a wide array of food matrices, while maintaining a high level of specificity in order to reduce the number of false positives. Due to our novel enrichment and lysis systems, the Polyskope 1.0 kit allows the simultaneous detection of any, or all three, of the major pathogens in a single overnight enrichment, lysis, and PCR reaction. This directly results in a 3-fold reduction in labor, media/reagent costs, regulatory burden, sample prep, media prep, laboratory space required, disposables used, and equipment required while still delivering results in 24 hours.

Available Q3 2017 – AOAC PTM Approval Pending

Inquire for information & demonstrations for your lab.